Electrochemical Aptasensor Based on ZnO-Au Nanocomposites for the Determination of Ochratoxin A in Wine and Beer

Ochratoxin A (OTA) is positively correlated with an increased risk of developing cancer in nephrotoxic and hepatotoxic patients. Therefore, it great significance for the highly sensitive, selective, timely detection OTA. We described here electrochemical aptasensor OTA analysis, which took advantage favorable properties gold nanoparticles (AuNPs) functionalized zinc oxide (ZnO) composites intercalative binding between methylene blue (MB) nucleic acid. There were two label-free aptamers: one to capture another serving as complementary DNA (cDNA), enabling connection ZnO-Au composite’s immobilized electrode. Once was present, aptamer could detach from electrode interface, thus, preventing MB accessing surface efficient electron transfer; a decreased peak current monitored by differential pulse voltammetry. The presented nice analytical performance range 0.1–30,000 pg·mL−1, limit 0.05 pg·mL−1. Moreover, developed biosensor be applied actual sample (wine beer) analysis.